National Cancer Institute Thesaurus

Last uploaded: February 21, 2019
Preferred Name

Polyacrylamide Gel Electrophoresis

ID

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C16539

ALT_DEFINITION

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

code

C16539

Concept_In_Subset

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C116977

Contributing_Source

CTRP

DEFINITION

Analytical and separative procedures in which molecules, particularly proteins or nucleic acids, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. For proteins, commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.

Display_Name

Polyacrylamide Gel Electrophoresis

FULL_SYN

PAGE

Fractionation, Polyacrylamide Gel

Electrophoresis, Polyacrylamide Gel

label

Polyacrylamide Gel Electrophoresis

Legacy_Concept_Name

Polyacrylamide_Gel_Electrophoresis

Preferred_Name

Polyacrylamide Gel Electrophoresis

prefixIRI

C16539

prefLabel

Polyacrylamide Gel Electrophoresis

Semantic_Type

Molecular Biology Research Technique

UMLS_CUI

C0013862

subClassOf

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C18091

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