National Cancer Institute Thesaurus

Last uploaded: February 21, 2019
Preferred Name

Electrophoretic Mobility Shift Assay

ID

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C19362

code

C19362

DEFINITION

A molecular biology technique used to detect the interaction of a DNA binding protein with its cognate binding sequence. Labeled DNAs were reacted with crude cell extracts and the complexes are run through a non-denaturing polyacrylamide gel. The migration of the labeled DNA through the gel will be slower by being bound. This shift in electrophoretic mobility indicates functional binding between the protein and the DNA.

FULL_SYN

Gel Shift Assay

EMSA

label

Electrophoretic Mobility Shift Assay

Legacy_Concept_Name

Electrophoretic_Mobility_Shift_Assay

Preferred_Name

Electrophoretic Mobility Shift Assay

prefixIRI

C19362

prefLabel

Electrophoretic Mobility Shift Assay

Semantic_Type

Laboratory Procedure

UMLS_CUI

C0949632

subClassOf

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C16539

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http://purl.obolibrary.org/obo/MI_0413 Molecular Interactions Controlled Vocabulary LOOM
http://purl.bioontology.org/ontology/MESH/D024202 Medical Subject Headings LOOM
http://purl.obolibrary.org/obo/OBI_0001671 Ontology for Biomedical Investigations LOOM