National Cancer Institute Thesaurus

Last uploaded: February 21, 2019
Preferred Name

Pyrosequencing

ID

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C99481

ALT_DEFINITION

A DNA sequencing technique that is based on the detection of released pyrophosphate during DNA synthesis.

code

C99481

Concept_In_Subset

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C66830

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C61410

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C85492

Contributing_Source

CDISC

DEFINITION

A DNA sequencing method that uses a "sequencing by synthesis" strategy. The strand complementary to a template is synthesized a single base at a time through the sequential addition of individual solutions of each base. Incorporation of a base into the growing strand generates free pyrophosphate that is detected as light, as generated by a luciferase-catalyzed reaction.

label

Pyrosequencing

Preferred_Name

Pyrosequencing

prefixIRI

C99481

prefLabel

Pyrosequencing

Semantic_Type

Molecular Biology Research Technique

UMLS_CUI

C2737269

subClassOf

http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C18881

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http://purl.obolibrary.org/obo/OBI_0000730 GenEpiO LOOM