loading...| Preferred Name |
enzyme-linked immunosorbent assay |
| Definitions |
An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte. |
| ID |
http://purl.obolibrary.org/obo/OBI_0000661 |
| alternative term |
ELISA |
| definition source |
IEDB |
| editor preferred label |
enzyme-linked immunosorbent assay |
| example of usage |
1) Detection of IL-2 (analyte) in a cell supernatant (evaluant), using plate bound anti IL-2 antibodies, and a reporter enzyme-linked reporter antibody. 2) Measurement of IgG antibody (analyte) titer in a serum sample (evaluant) using plate bound antigen and a reporter anti-IgG antibody. |
| has curation status | |
| label |
enzyme-linked immunosorbent assay |
| prefixIRI |
OBI:0000661 |
| prefLabel |
enzyme-linked immunosorbent assay |
| term editor |
IEDB |
| textual definition |
An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte. |
| subClassOf |
| Delete | Mapping To | Ontology | Source |
|---|---|---|---|
| http://purl.obolibrary.org/obo/MI_0411 | Molecular Interactions Controlled Vocabulary | LOOM | |
| http://purl.bioontology.org/ontology/MESH/D004797 | Medical Subject Headings | LOOM |