Ontology for Biomedical Investigations

Last uploaded: January 23, 2019
Preferred Name

electrophoretic mobility shift assay

Definitions

An assay that measures information about Protein-DNA or Protein-RNA interactions using gel electrophoresis and relying on the fact the molecular interactions will cause the heterodimer to be retarded on the gel when compared to controls corresponding to protein extract alone and protein extract + neutral nucleic acid.

ID

http://purl.obolibrary.org/obo/OBI_0001671

alternative term

gel mobility shift assay

gel retardation assay

band shift assay

gel shift assay

EMSA

definition source

PMID:6269071

editor preferred label

electrophoretic mobility shift assay

example of usage

Electrophoretic mobility shift assay reveals a novel recognition sequence for Setaria italica NAC protein. PMID:21918373

has curation status

http://purl.obolibrary.org/obo/IAO_0000122

has_specified_input

http://purl.obolibrary.org/obo/OBI_0000880

has_specified_output

http://purl.obolibrary.org/obo/IAO_0000027

label

electrophoretic mobility shift assay

prefixIRI

OBI:0001671

prefLabel

electrophoretic mobility shift assay

term editor

Philippe Rocca-Serra

Marcus Chibucos

textual definition

An assay that measures information about Protein-DNA or Protein-RNA interactions using gel electrophoresis and relying on the fact the molecular interactions will cause the heterodimer to be retarded on the gel when compared to controls corresponding to protein extract alone and protein extract + neutral nucleic acid.

subClassOf

http://purl.obolibrary.org/obo/OBI_0001146

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Delete Mapping To Ontology Source
http://purl.obolibrary.org/obo/MI_0413 Molecular Interactions Controlled Vocabulary LOOM
http://purl.bioontology.org/ontology/MESH/D024202 Medical Subject Headings LOOM
http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C19362 National Cancer Institute Thesaurus LOOM