loading...| Preferred Name |
electrophoretic mobility shift assay |
| Synonyms |
band shift emsa Gel retardation assay |
| Definitions |
This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed. |
| ID |
http://purl.obolibrary.org/obo/MI_0413 |
| definition |
This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed. |
| has_exact_synonym |
band shift emsa Gel retardation assay |
| has_obo_namespace |
PSI-MI |
| id |
MI:0413 |
| in_subset | |
| label |
electrophoretic mobility shift assay |
| notation |
MI:0413 |
| prefLabel |
electrophoretic mobility shift assay |
| treeView | |
| subClassOf |