Ontology for Biomedical Investigations

Last uploaded: January 23, 2019
Preferred Name

enzyme-linked immunosorbent assay

Definitions

An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte.

ID

http://purl.obolibrary.org/obo/OBI_0000661

alternative term

ELISA

definition source

IEDB

editor preferred label

enzyme-linked immunosorbent assay

example of usage

1) Detection of IL-2 (analyte) in a cell supernatant (evaluant), using plate bound anti IL-2 antibodies, and a reporter enzyme-linked reporter antibody. 2) Measurement of IgG antibody (analyte) titer in a serum sample (evaluant) using plate bound antigen and a reporter anti-IgG antibody.

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

label

enzyme-linked immunosorbent assay

prefixIRI

OBI:0000661

prefLabel

enzyme-linked immunosorbent assay

term editor

IEDB

textual definition

An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte.

subClassOf

http://purl.obolibrary.org/obo/OBI_0000443

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Delete Mapping To Ontology Source
http://purl.obolibrary.org/obo/MI_0411 Molecular Interactions Controlled Vocabulary LOOM
http://purl.bioontology.org/ontology/MESH/D004797 Medical Subject Headings LOOM