enzyme-linked immunospot assay

A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.

http://purl.obolibrary.org/obo/OBI_0600031

ELISPOT assay

IEDB

enzyme-linked immunospot assay

Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell.

http://purl.obolibrary.org/obo/IAO_0000120

enzyme-linked immunospot assay

OBI:0600031

enzyme-linked immunospot assay

IEDB

A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.

http://purl.obolibrary.org/obo/OBI_0001977